I found that disabling Z-Hop either globally or in the material setting overrides (setting Z-Hop to 0) leads to the wrong nozzle height on the first layer, if the object is preceded by a prime tower. Weighing both the faulty and intact prints of a single layer shows identical weight of both, clearly indicating that this is NOT an underextrusion problem. Measuring the test body height reveals a first layer height of 0.6 mm instead of the required 0.25. The issue is fully repeatable across both nozzles and materials and can be toggled reliably with the z-hop setting. The G-Code of defective and intact prints appears to be identical, suggesting that the issue originates somewhere in the accessory files of the g-code.3mf file and firmware interpretation.
Bambu Studio Version 2.4.0.70
Printer: H2D
FW Version: 01.02.02.00

