Inconsistent surface quality on the same print

What’s causing these differences in top surface quality? Its the same print so i would think it “treated” the two top surfaces the same.

I’m using a A1 mini with calibrated esun matte filament. I have never had a problem with this filament or type of print before

A few things. Pretty sure there are separate settings in Studio for the actual topmost layer and other, intermediate top layers. Have you checked that those both are set the same? And maybe something like ironing could help if you want a smoother finish?

And there can be circumstances that can put you close enough to having print issues that they start to appear just on parts of the print and not all of it. Especially with open printers, air currents can cool parts of a print, or humidity or water content in the filament can cause issues on tougher parts of the print. If things are borderline, little things can seem to have outsized effects.